Synthesising Psorinum 1M Nanoparticle by chemical Method

Dr Suraj Singh Bhadoria 1, Dr Sandhya Vasant Gharge 2

Abstract
Through this research work synthesizing the nanoparticles in Psorinum 1M by Potassium Ferricynaide as a biocatalyst and inclusivity of succussion potentization method. After analysis under SEM (Scanning electron microscope), minute strictures of massless particles nanoparticles synthesized successfully.

Keywords: nanoparticles, Psorinum, potentization, biocatalyst

Introduction
Particulate dispersions or solid particles with a size between 10 and 1000 nm are referred to as nanoparticles. The medication dissolves, imprisoned, enclosed, or affixed to a matrix of nanoparticles. Nanoparticles, nanospheres, or nanocapsules can be produced based on the preparation technique. Nanospheres are matrix systems where the drug is uniformly and physically distributed, whereas nanocapsules are systems where the medication is contained within a hollow encircled by a special polymer membrane. Biodegradable polymeric nanoparticles, especially those coated with hydrophilic polymers like poly(ethylene glycol) (PEG), also referred to as long-circulating particles, have been explored as possible drug delivery vehicles in recent years due to their capacity to target a specific organ, circulate for an extended amount of time, and act as DNA carriers in gene therapy. and their capacity to transfer genes ^[1-4], proteins, and peptides.

In order to accomplish the site-specific action of the drug at the therapeutically optimal rate and dose regimen, the main objectives of designing nanoparticles as a delivery system are to manage particle size, surface characteristics, and release of pharmacologically active substances.
Although liposomes have been employed as potential carriers with special benefits like preventing drug degradation, directing medication to the site of action, and lowering toxicity or adverse effects, their uses are restricted because of innate issues like poor storage stability, low encapsulation efficiency, and rapid leakage of water-soluble drugs in the presence of blood components. However, compared to liposomes, polymeric nanoparticles have a few unique benefits. For example, they have beneficial controlled release qualities and aid in improving the stability of medications and proteins. ^[5,6]

Materials & Methodology

Type of study: Analytical study

Site of Study: Homoeopathic Pharmacy laboratory of Paul Institute of Homoeopathy & Research, Parul University, Vadodara, Sophisticated Instrumentation Centre for Applied Research and Testing – SICART Sponsored by Department of Science and Technology, Govt. of India, New Delhi Sardar Patel Centre for Science and Technology Behind V.P. & R.P.T.P. Science College Charutar Vidya Mandal Vallabh Vidyanagar.

Medicinal Product: Psorinum

Potency: 1M

Instruments: Hot air oven, Electric Potentizer Machine, Measuring cylinder 10 ml capacity, electronic weight machine, Glass beaker 50 ml capacity, spatula, glass rod

Investigation tool: SEM Scanning electron microscope

Model: Nova NanoSEM 450

Preparations:

1^St step: sterilization of all the laboratory equipment’s like Measuring cylinder 10 ml capacity, Glass beaker 50 ml capacity, glass rod in Hot air oven.

2^nd step: While preparing the nanoparticles in psorium 1M, took 1 ml psorinum 1M in 20 ml distilled water along with 5 gm potassium Ferricynaide as a biocatalyst, which are helpful in synthesizing nanostructures.

3^rd step: Filling of suspension in sterile bottle 100 ml capacity.

4^th step: succussion done up to 100 times by electric potentizer machine

5^th step: sample should e analyse under SEM Scanning electron microscope

Results:
The SEM Analysis of Psoinum 1M is given below;

Conclusion
After analysis under SEM (Scanning electron microscope), minute strictures of massless particles nanoparticles synthesized successfully.

Acknowledgment
Authors would like to thanks Parul University and Sophisticated Instrumentation Centre for Applied Research and Testing for constant support to complete this research work.

References

1. Langer R. Biomaterials in drug delivery and tissue engineering: one laboratory’s experience. Acc Chem Res 2000; 33: 94-101.
2. Bhadra D, Bhadra S, Jain P, Jain NK. Pegnology: a review of PEG-ylated systems. Pharmazie 2002; 57: 5-29.
3. Kommareddy S, Tiwari SB, Amiji MM. Long-circulating polymeric nanovectors for tumor-selective gene delivery. Technol Cancer Res Treat 2005; 4: 615- 25.
4. Lee M, Kim SW. Polyethylene glycol-conjugated copolymers for plasmid DNA delivery. Pharm Res 2005; 22: 1-10.
5. Vila A, Sanchez A, Tobio M, Calvo P, Alonso MJ. Design of biodegradable particles for protein delivery. J Control Release 2002; 78: 15-24.
6. Mu L, Feng SS. A novel controlled release formulation for the anticancer drug paclitaxel (Taxol(R)): PLGA nanoparticles containing vitamin E TPGS. J Control Release 2003; 86: 33-48.

Dr Suraj Singh Bhadoria 1, Dr Sandhya Vasant Gharge 2

1. Assistant professor in the Department of Homoeopathic Pharmacy (PG), Parul Institute of Homoeopathy & Research, Parul University, Vadodara, Gujarat
2. Assistant professor in Department of Obstetrics & Gynaecology, Bharati Vidyapeeth (Deemed to be University) Homoeopathic Medical College, Pune, Maharasthra
   Email ID: drsurajsinghbhadoria29@gmail.com

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