Introduction: Nosodes are homoeopathic preparations sourced from biological materials such diseased tissues, organisms, cultures (bacteria, fungi, and viruses) or parasites, or from decomposed products from humans or animals. More than forty‑five major nosodes have been in use since 1830 but no clear guidelines regarding their preparation are available.
Objective: To standardize the method of preparation of nosodes using modern technology and lay down clear guidelines for the same.
Materials and Methods : Biological material identifcation such as culture of organism, separation of required pure fraction, quantifcation, standardization, dilutions, potentisation method, and safe use of nosodes were documented in a systematic way .
Result: HIV, HCV and Mycobacterium tuberculosis nosodes were prepared using the modifed method described in this article.
Conclusion: The improved method of nosode preparation can pave way for laying clear guidelines for preparation of nosodes in homoeopathic pharmacopeias.
Keywords: Ethics, Human immunodefciency virus, Hepatitis C, Method, Mycobacterium tuberculosis, Nosode, Organism, Potentization, Standardisation